RNA folding at elementary step resolution

RNA folding at elementary step resolution.

We study the stochastic folding kinetics of RNA sequences into secondary structures with a new algorithm based on the formation, dissociation, and the shifting of individual base pairs. We discuss folding mechanisms and the correlation between the barrier structure of the conformational landscape and the folding kinetics for a number of examples based on artificial and natural sequences, includ...

ReviewProtein Folding and Unfolding at Atomic Resolution

resolution. The relevance of folding in vitro to folding in vivo was established using two small proteins, CI2 (chymotrypsin inhibitor 2, 64 residues) and barnase (BaAlan R. Fersht1,3 and Valerie Daggett2,3 1 Department of Chemistry and MRC Centre for Protein Engineering cillus amylolquiefaciens Rnase, 110 residues), the two University of Cambridge proteins on which many of the modern methods o...

Protein Folding and Unfolding at Atomic Resolution

Experiment and simulation are now conspiring to give atomic-level descriptions of protein folding relevant to folding, misfolding, trafficking, and degradation in the cell. We are on the threshold of predicting those protein folding events using simulation that has been carefully benchmarked by experiment.

Beyond static structures of RNA by NMR: folding, refolding, and dynamics at atomic resolution.

Time-resolved NMR methods resolving ligand-induced RNA folding at atomic resolution.

Structural transitions of RNA between alternate conformations with similar stabilities are associated with important aspects of cellular function. Few techniques presently exist that are capable of monitoring such transitions and thereby provide insight into RNA dynamics and function at atomic resolution. Riboswitches are found in the 5'-UTR of mRNA and control gene expression through structura...